Fig. 4.

Intrinsic torque jump of F₁. Data points of angular displacement (θ_B − θ_TC) obtained from controlled rotation to the ATP hydrolysis direction (0.04 Hz, six revolutions) are shown in 120° unit (gray points), and filtered by its density (yellow points) these are almost same procedures as steps 1–3 in Fig. S2. The extracted dense points are median-filtered with 500 points without clustering process (black line): (A) ATP, ADP, and P_i = 10, 10, and 100 μM (condition i); (B) 0, 10, and 100 μM; (C) 0, 10, and 0 μM; (D) 0, 0, and 100 μM; and (E) no substrate. The change of substrate was achieved by exchange of the solution in the observation chamber. These data were obtained from one of three F₁ molecules used in the torque quantification of this research. (F) Intrinsic torque jump and transition between states I and II. Torque traces in A–E were overlaid.