Table S1.

Variants of the subglobal fits to the two-state model in which clusters of residues are constrained to have the same values of k_ON and k_OFF

					Fitted parameter values					Correlation between ΔωFB and ΔδFB
Fit type	Global parameters, in addition to cM and cK	Local parameters, in addition to ΔωFB and R20	No. parameters	Reduced χ2	Average kON (× 107 M−1⋅s−1)	N-terminal average kOFF, s−1*	C-terminal average kOFF, s−1†	cM	cK	Slope	r‡
1§	—	kON, kOFF	132	2.097	1.71 ± 0.05	16.6 ± 0.7	12.7 ± 0.4	1.030	0.959	1.01 ± 0.08	0.973
2a	kON1(290–303), kON2(305–309)	kOFF	122	2.114	1.75 ± 0.02	16.4 ± 0.6	12.8 ± 0.4	1.034	0.963	1.00 ± 0.07	0.974
2b	kON1(290–301), kON2(302–309)	kOFF	122	2.117	1.65 ± 0.02	16.4 ± 0.6	12.8 ± 0.4	1.070	0.997	1.00 ± 0.08	0.973
3	kON(290–309)	kOFF	121	2.120	1.70 ± 0.04	16.3 ± 0.6	12.8 ± 0.4	1.039	0.967	1.00 ± 0.07	0.975
4a	kOFF1(290–303), kOFF2(305–309)	kON	122	2.154	1.70 ± 0.06	15.8 ± 0.2	12.4 ± 0.2	1.037	0.966	1.01 ± 0.06	0.981
4b	kOFF1(290–301), kOFF2(302–309)	kON	122	2.149	1.81 ± 0.06	15.8 ± 0.2	12.6 ± 0.2	1.027	0.954	0.97 ± 0.07	0.975
5a	kON(290–309), kOFF1(290–303), kOFF2(305–309)	—	111	2.168	1.73 ± 0.04	15.8 ± 0.2	12.5 ± 0.2	1.036	0.964	0.99 ± 0.06	0.983
5b	kON(290–309), kOFF1(290–301), kOFF2(302–309)	—	111	2.167	1.80 ± 0.04	16.0 ± 0.2	12.5 ± 0.2	1.021	0.950	0.97 ± 0.07	0.973
6a	kON1(290–303), kON2(305–309), kOFF1(290–303), kOFF2(305–309)	—	112	2.171	1.65 ± 0.04	15.8 ± 0.6	12.6 ± 0.7	1.056	0.983	1.00 ± 0.06	0.981
6b	kON1(290–301), kON2(302–309), kOFF1(290–301), kOFF2(302–309)	—	112	2.175	1.75 ± 0.04	16.1 ± 0.7	12.6 ± 0.7	1.040	0.967	0.97 ± 0.08	0.969
7	kOFF(290–309)	kON	121	2.380	2.02 ± 0.09	13.7 ± 0.1		0.989	0.917	0.93 ± 0.04	0.992
8	kON(290–309), kOFF(290–309)	—	110	2.409	2.18 ± 0.06	13.6 ± 0.1		0.960	0.889	0.91 ± 0.03	0.993

^*k_OFF1 or the average of k_OFF for residues 290–301.

^†k_OFF2 or the average of k_OFF for residues 302–309.

^‡Correlation coefficient.

^§The results of this type of fit are shown in Fig. 2.