Fig. S1.

AIS plasticity after chronic stimulation leads to reduced intrinsic excitability. (A) Current clamp recordings were taken from ChR2-stimulated neurons (ChR2-stimulated), neighboring untransfected neurons (Neighbor), and ChR2-transfected but unstimulated neurons (ChR2). Sample responses to 500-ms current injections of varying strength shown below. (B) Voltage threshold and current threshold density for 10-ms and 500-ms current injections (n = 16 neighboring, n = 14 ChR2, n = 14 ChR2-stimulated cells). (C) Input-output curves in response to 500-ms current injections of increasing strength. All plots show mean ± SEM; **P < 0.01, ***P < 0.001.