1C (AIS length) |
Mann–Whitney U
|
0.4 |
17 CA3 cells, 16 CA1 cells |
1C (No. of VGAT puncta) |
Mann–Whitney U
|
0.015 |
12 CA3 cells, 16 CA1 cells |
2C (Distance from axon origin) |
Mann–Whitney U
|
0.007 start, 0.015 max, 0.042 end |
15 GFP cells, 16 ChR2-stimulated cells |
2C (Distance from soma) |
Mann–Whitney U
|
0.007 start, 5.43 × 10−4 max; 0.004 end |
15 GFP cells, 16 ChR2-stimulated cells |
2D
|
Student’s t test |
0.32 |
15 GFP cells, 16 ChR2-stimulated cells |
2F (Position from soma) |
Mann–Whitney U
|
0.86 |
136 Synapses, 14 cells control; 109 synapses, 14 cells ChR2-stimulated |
2F (Position relative to AIS) |
Mann–Whitney U
|
1.8 × 10−4
|
136 Synapses, 14 cells control; 109 synapses, 14 cells ChR2-stimulated |
2G (Position from soma) |
Mann–Whitney U
|
0.29 |
207 Synapses, 14 cells control; 183 synapses, 14 cells ChR2-stimulated |
2G (Position relative to AIS) |
Mann–Whitney U
|
5.93 × 10−8
|
207 Synapses, 14 cells control; 183 synapses, 14 cells ChR2-stimulated |
2H (VGAT) |
Mann–Whitney U
|
9.91 × 10−4 Before AIS; 0.032 on AIS |
14 Control cells, 14 ChR2-stimulated cells |
2H (GABAAα2) |
Mann–Whitney U
|
0.0013 Before AIS; 0.89 on AIS |
14 Control cells, 14 ChR2-stimulated cells |
S1B (Voltage threshold 10 ms) |
One-way ANOVA with Tukey–Kramer post hoc tests |
Population: 0.011 |
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: 0.011 ChR2 stim vs. NBQX; 0.64 ChR2-stimulated vs. ChR2; 0.1 ChR2 vs. neighbor |
S1B (Voltage threshold, 500 ms) |
One-way ANOVA with Tukey–Kramer post hoc tests |
Population: 0.005 |
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: 0.004 ChR2-stimulated vs. neighbor; 0.45 ChR2-stimulated vs. ChR2; 0.1 ChR2 vs. neighbor |
S1B (Current threshold density, 10 ms) |
One-way ANOVA with Tukey–Kramer post hoc tests |
Population: 1.54 × 10−6
|
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: |
1.02 × 10−5 ChR2-stimulated vs. neighbor; 0.0002 ChR2-stimulated vs. ChR2; 0.4 ChR2 vs. neighbor |
S1B (Current threshold density 500 ms) |
Kruskal–Wallis ANOVA with Dunn post hoc tests |
Population: 1.26 × 10−5
|
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: 1.4 × 10−5 ChR2-stimulated vs. neighbor; 0.0018 ChR2-stimulated vs. ChR2; 0.56 ChR2 vs. neighbor |
S2A
|
Kruskal–Wallis ANOVA |
0.16 |
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
S2B
|
Kruskal–Wallis ANOVA with Dunn post hoc tests |
Population: 9.6 × 10−4
|
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: 0.0008 ChR2-stimulated vs. neighbor; 0.034 ChR2-stimulated vs. ChR2; 0.51 ChR2 vs. neighbor |
S2C (No drug) |
One-way ANOVA with Tukey–Kramer post hoc tests |
Population: 2.32 × 10−4
|
16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
Pairwise comparisons: 0.0004 ChR2-stimulated vs. neighbor; 0.0024 ChR2-stimulated vs. ChR2; 0.86 ChR2 vs. neighbor |
S2C (+Picrotoxin) |
Student’s t test |
0.043 |
9 Neighboring cells; 8 ChR2-stimulated cells |
S2C (+ZD7288) |
Student’s t test |
2.86 × 10−4
|
4 Neighboring cells; 7 ChR2-stimulated cells |
S2C (+BaCl2) |
Mann–Whitney U
|
0.008 |
6 Neighboring cells; 7 ChR2-stimulated cells |
S3A
|
Student’s t test |
6.43 × 10−6 neighbor vs. ChR2-stimulated; 0.18 neighbor vs. neighbor + PTX; 0.008 neighbor + PTX vs. ChR2-stimulated + PTX; 0.04 ChR2-stimulated vs. ChR2 stim + PTX |
16 Neighboring cells; 14 ChR2-stimulated cells; 9 neighboring cells + Picrotoxin; 8 ChR2-stimulated cells + Picrotoxin |
S3B
|
Mann–Whitney U
|
1.4 × 10−5 neighbor vs. ChR2-stimulated; 0.03 neighbor vs. neighbor + PTX; 8.6 × 10−4 neighbor + PTX vs. ChR2-stimulated + PTX; 0.002 ChR2-stimulated vs. ChR2-stimulated + PTX |
16 Neighboring cells; 14 ChR2-stimulated cells; 9 neighboring cells + Picrotoxin; 8 ChR2-stimulated cells + Picrotoxin |
S4 (VGAT) |
Mann–Whitney U
|
0.008 before AIS; 0.033 on AIS; 0.71 after AIS; 0.002 before vs. on AIS control; 0.017 before vs. after AIS control; 0.64 on vs. after AIS control; 0.21 before vs. on AIS ChR2-stimulated; 0.14 before vs. after AIS ChR2-stimulated; 0.63 on vs. after AIS ChR2-stimulated |
14 Control cells, 14 ChR2-stimulated cells |
S4 (GABAAα2) |
Mann–Whitney U
|
0.007 before AIS; 0.73 on AIS; 0.4 after AIS; 0.005 before vs. on AIS control; 0.016 before vs. after AIS control; 0.15 on vs. after AIS control; 0.48 before vs. on AIS ChR2-stimulated; 0.13 before vs. after AIS ChR2-stimulated; 0.25 on vs. after AIS ChR2-stimulated |
14 Control cells, 14 ChR2-stimulated cells |