Table S1.
Summary of statistical analyses
| Figure | Test | P value | Number |
| 1C (AIS length) | Mann–Whitney U | 0.4 | 17 CA3 cells, 16 CA1 cells |
| 1C (No. of VGAT puncta) | Mann–Whitney U | 0.015 | 12 CA3 cells, 16 CA1 cells |
| 2C (Distance from axon origin) | Mann–Whitney U | 0.007 start, 0.015 max, 0.042 end | 15 GFP cells, 16 ChR2-stimulated cells |
| 2C (Distance from soma) | Mann–Whitney U | 0.007 start, 5.43 × 10−4 max; 0.004 end | 15 GFP cells, 16 ChR2-stimulated cells |
| 2D | Student’s t test | 0.32 | 15 GFP cells, 16 ChR2-stimulated cells |
| 2F (Position from soma) | Mann–Whitney U | 0.86 | 136 Synapses, 14 cells control; 109 synapses, 14 cells ChR2-stimulated |
| 2F (Position relative to AIS) | Mann–Whitney U | 1.8 × 10−4 | 136 Synapses, 14 cells control; 109 synapses, 14 cells ChR2-stimulated |
| 2G (Position from soma) | Mann–Whitney U | 0.29 | 207 Synapses, 14 cells control; 183 synapses, 14 cells ChR2-stimulated |
| 2G (Position relative to AIS) | Mann–Whitney U | 5.93 × 10−8 | 207 Synapses, 14 cells control; 183 synapses, 14 cells ChR2-stimulated |
| 2H (VGAT) | Mann–Whitney U | 9.91 × 10−4 Before AIS; 0.032 on AIS | 14 Control cells, 14 ChR2-stimulated cells |
| 2H (GABAAα2) | Mann–Whitney U | 0.0013 Before AIS; 0.89 on AIS | 14 Control cells, 14 ChR2-stimulated cells |
| S1B (Voltage threshold 10 ms) | One-way ANOVA with Tukey–Kramer post hoc tests | Population: 0.011 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: 0.011 ChR2 stim vs. NBQX; 0.64 ChR2-stimulated vs. ChR2; 0.1 ChR2 vs. neighbor | |||
| S1B (Voltage threshold, 500 ms) | One-way ANOVA with Tukey–Kramer post hoc tests | Population: 0.005 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: 0.004 ChR2-stimulated vs. neighbor; 0.45 ChR2-stimulated vs. ChR2; 0.1 ChR2 vs. neighbor | |||
| S1B (Current threshold density, 10 ms) | One-way ANOVA with Tukey–Kramer post hoc tests | Population: 1.54 × 10−6 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: | |||
| 1.02 × 10−5 ChR2-stimulated vs. neighbor; 0.0002 ChR2-stimulated vs. ChR2; 0.4 ChR2 vs. neighbor | |||
| S1B (Current threshold density 500 ms) | Kruskal–Wallis ANOVA with Dunn post hoc tests | Population: 1.26 × 10−5 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: 1.4 × 10−5 ChR2-stimulated vs. neighbor; 0.0018 ChR2-stimulated vs. ChR2; 0.56 ChR2 vs. neighbor | |||
| S2A | Kruskal–Wallis ANOVA | 0.16 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| S2B | Kruskal–Wallis ANOVA with Dunn post hoc tests | Population: 9.6 × 10−4 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: 0.0008 ChR2-stimulated vs. neighbor; 0.034 ChR2-stimulated vs. ChR2; 0.51 ChR2 vs. neighbor | |||
| S2C (No drug) | One-way ANOVA with Tukey–Kramer post hoc tests | Population: 2.32 × 10−4 | 16 Neighboring cells, 14 ChR2 cells, 14 ChR2-stimulated cells |
| Pairwise comparisons: 0.0004 ChR2-stimulated vs. neighbor; 0.0024 ChR2-stimulated vs. ChR2; 0.86 ChR2 vs. neighbor | |||
| S2C (+Picrotoxin) | Student’s t test | 0.043 | 9 Neighboring cells; 8 ChR2-stimulated cells |
| S2C (+ZD7288) | Student’s t test | 2.86 × 10−4 | 4 Neighboring cells; 7 ChR2-stimulated cells |
| S2C (+BaCl2) | Mann–Whitney U | 0.008 | 6 Neighboring cells; 7 ChR2-stimulated cells |
| S3A | Student’s t test | 6.43 × 10−6 neighbor vs. ChR2-stimulated; 0.18 neighbor vs. neighbor + PTX; 0.008 neighbor + PTX vs. ChR2-stimulated + PTX; 0.04 ChR2-stimulated vs. ChR2 stim + PTX | 16 Neighboring cells; 14 ChR2-stimulated cells; 9 neighboring cells + Picrotoxin; 8 ChR2-stimulated cells + Picrotoxin |
| S3B | Mann–Whitney U | 1.4 × 10−5 neighbor vs. ChR2-stimulated; 0.03 neighbor vs. neighbor + PTX; 8.6 × 10−4 neighbor + PTX vs. ChR2-stimulated + PTX; 0.002 ChR2-stimulated vs. ChR2-stimulated + PTX | 16 Neighboring cells; 14 ChR2-stimulated cells; 9 neighboring cells + Picrotoxin; 8 ChR2-stimulated cells + Picrotoxin |
| S4 (VGAT) | Mann–Whitney U | 0.008 before AIS; 0.033 on AIS; 0.71 after AIS; 0.002 before vs. on AIS control; 0.017 before vs. after AIS control; 0.64 on vs. after AIS control; 0.21 before vs. on AIS ChR2-stimulated; 0.14 before vs. after AIS ChR2-stimulated; 0.63 on vs. after AIS ChR2-stimulated | 14 Control cells, 14 ChR2-stimulated cells |
| S4 (GABAAα2) | Mann–Whitney U | 0.007 before AIS; 0.73 on AIS; 0.4 after AIS; 0.005 before vs. on AIS control; 0.016 before vs. after AIS control; 0.15 on vs. after AIS control; 0.48 before vs. on AIS ChR2-stimulated; 0.13 before vs. after AIS ChR2-stimulated; 0.25 on vs. after AIS ChR2-stimulated | 14 Control cells, 14 ChR2-stimulated cells |