Fig. 3.

Analyses of MVA-induced nuclear-associated Ca²⁺ spiking responses in symbiosis-defective mutants of M. truncatula. (A) MVA-induced Ca²⁺ spiking was not observed in an nfp mutant, which acts upstream of DMI2. (B) MVA activates Ca²⁺ spiking in the dmi2 mutant expressing YC3.6, (C) but not in the dmi1 mutant. (D) As expected, MVA-elicited Ca²⁺ spiking is not modified in the dmi3 mutant, as DMI3 acts downstream of the Ca²⁺ spiking machinery.