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Circadian expression pattern of PhLHY and intracellular localization of PhLHY protein. (A and B) Expression profiles of PhLHY and PhGI in continuous dark over 92 h in Petunia petals (A) and leaves (B). Results represent means ± SEM from three biological replicates. (C–E) PhLHY-GFP is a nuclear localized protein. PhLHY-GFP protein (C) and H2B-RFP protein (reference for nuclei) (D) were expressed in epidermal cells of Petunia petals. (E) Merged image of C and D. (Scale bar: 10 μm.)
