Fig. 3.

Several ZFP regulate the expression of TNF through its 3′UTR. (A) Schematic representation of the GFP reporter constructs used to assay the effect of ZFP. (B) Representative FACS data showing the effect of the TNF 3′UTR on the expression of a GFP reporter, its down-regulation by Zfp36, and its up-regulation by Zc3h10. (C) Schematic representation of the deletions in the reporters used to define the regulatory regions in the TNF 3′UTR. (D) Regions 4 and 6 contain elements through which Zfp36, Zc3h12a, Zc3h12c, and Mkrn1 exert their regulatory function. (E) Strong synergy between regulatory regions 4 and 6. Data in D and E represent mean ± SEM of three independent experiments.