Fig. S4.
γδ-17 precursors express IL-15Rα. Neonatal thymi were harvested from C57BL/6-Il17atm1Bcgen/J pups within the first 24 h after birth. (A) Representative dot plot showing CD44 expression vs. GFP/IL17 production in the CD3+GL3+TCRβ− γδ population. (B) Representative histograms depicting IL-15Rα expression on each of the indicated quadrants from A. The gray- filled histograms show IL-15Rα expression in the negative control sample, which lacked the primary IL-15Rα–biotin antibody but included the secondary streptavidin-PE-Texas red detection reagent. (C) The average MFI of IL-15Rα for each population. One-way ANOVA was used to determine significance. Data are representative of two independent experiments with nine neoThy per experiment.