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. 2015 Jul 20;112(31):9686–9691. doi: 10.1073/pnas.1503535112

Fig. 3.

Fig. 3.

CME of MACs is required for NIK stabilization. (A) HUVECs treated with control, clathrin, or AP2 siRNA were analyzed for C9 colocalization with Rab5. (B) Western blot analysis of ECs treated with LIGHT (100 ng/mL) or PRA (1:5 dilution) for 24 h or 4 h, respectively, in the presence of control, clathrin, or AP2 siRNA as indicated. (C) Following treatment with control or dynamin siRNA, colocalization between C9 and Rab5 was quantified. (D) ECs were treated with dynasore, DNM2 siRNA, or DNM2 DN or the relevant control and analyzed after 4 h of PRA treatment. Each experiment was repeated two to four times, with similar results. Quantitation of the data in A and C are represented as mean ± SEM. *P < 0.05. (Scale bars: 10 μm.)