Fig. S2.

A genome-wide siRNA screen identifies vesicular trafficking genes involved in MAC-induced NIK stabilization. (A and B) ECs stably transduced with an NF-κB luciferase reporter were treated for 6 h as indicated. (C) Strategy for siRNA screening of PRA-treated ECs. (D) Genome-wide siRNA screening was performed twice. Pearson's correlation coefficient was calculated (r = 0.86), and genes with ≥2 SD robust z-scores were analyzed manually. (E and F) Selected genes were counterscreened with TNF-α (E), and genes with a average reduction in luciferase activity of PRA ≥50% and TNF-α ≤50% were subjected to pathway analysis and to deconvolution experiments in which individual siRNAs targeting unique exon regions from the original siRNA pool for each gene were tested in duplicate (F).