Fig. S7.
Dynasore inhibits noncanonical NF-κB–dependent Inflammatory genes in vitro and blocks clathrin-mediated endocytosis in vivo. HUVECs carrying an NF-κB–responsive luciferase reporter were pretreated for 30 min with vehicle, dynasore (80 μM), or pitstop 2 (30 μM), followed by exposure to PRA for 6 h, followed by analysis for luciferase activity (A) or for 4 h before assessment for NIK-dependent inflammatory gene induction (B). Results are expressed in cycle time normalized to GAPDH expression, where a decrease in cycle time by x indicates an increase in gene expression by 2x. (C) Grafts were treated with vehicle or dynasore before i.v. injection of PRA and DiI-LDL, a ligand used for clathrin-mediated endocytosis. *P < 0.05. n = 3 pairs of mice. (Scale bar: 100 μm.)