Fig. 8.

Knock-off analysis indicates tethering of Xist and PRC2 during dissociation and relocalization, supporting a comigration along the Xi. (A) Representative STORM images of Xist and EZH2 immuno RNA FISH are shown at various time points after LNA treatment to knock off Xist-PRC2 complexes. (B) The percentage of nearest neighbors located within 50 nm of each other is shown, and differences between values for the observed (O) versus randomized (R) models at each time point were tested for statistical significance (Student’s two-tailed t test). (C) Summary: High-resolution single-cell view of Xist on the Xi, versus epigenomic profiling across a population. ChIP-seq (11) and CHART-seq (10) data as published. (D) One possible hit-and-run model: Xist-PRC2 complexes are anchored at secondary sites and serially methylate nucleosomes, looping out chromatin as it processes locally along the Xi.