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3D STORM imaging of Xist RNA is not affected by various fixation methods. (A) Cells were fixed in PFA and then permeablized in Triton-X 100 before RNA FISH. (B) Cells were preextracted in CSK + 0.5% Triton-X 100 before fixation in PFA. (C) Cells were fixed and permeablized in cold MeOH. (D) Cells were fixed and permeablized in cold Carnoy fixative (MeOH:acetic acid = 3:1). Depth in the z-plane was color-coded from red (+400 nm) to green (−400 nm).
