Fig. 2.

Inhibition of plasma ADAMTS13 activity by scFvs. Residual activity of plasma-derived ADAMTS13 (∼0.25 nM) after incubation with increasing concentrations of scFvs 4–20 (A), 4–16 (B), and 3–1 (C) was determined by cleavage of the fluorogenic substrate (rF-VWF73) (2 μM) as described in SI Materials and Methods. Data are the mean ± SD of three independent experiments.