Table S1.
H-D exchange protection factor (Pf) and Pf increase when bound to scFvs
Residue | AA | Switch | NoAb Pf | scFv4-20 Pf | scFv4_20 ratio | scFv4_16 Pf | scFv4_16 ratio | scFv3-1 Pf | scFv3-1 ratio |
585 | I | 0 | >7E+5 | >7E+5 | — | >7E+5 | — | >7E+5 | — |
586 | A | 0 | >7E+5 | >7E+5 | — | >7E+5 | — | >7E+5 | — |
587 | N | 1 | >7E+5 | >7E+5 | — | >7E+5 | — | >7E+5 | — |
588 | H | 1 | 2.E+04 | 3.E+05 | 15 | 2.E+05 | 10 | 8.E+04 | 4 |
589 | R | 1 | 2.E+04 | 1.E+05 | 5 | 5.E+04 | 3 | 4.E+05 | 20 |
591 | L | 1 | 3.E+03 | 8.E+04 | 27 | 2.E+04 | 7 | 9.E+04 | 30 |
592 | F | 1 | <300 | 4.E+03 | >13 | 4.E+03 | >13 | 6.E+04 | >200 |
593 | T | 2 | >7E+5 | >7E+5 | — | >7E+5 | — | >7E+5 | — |
594 | H | 2 | 5.E+05 | >7E+5 | >1 | >7E+5 | >1 | >7E+5 | >1 |
595 | L | 2 | <300 | 1.E+03 | >3 | <300 | — | 1.E+03 | >3 |
598 | R | 3 | 7.E+05 | 8.E+05 | 1 | 9.E+05 | 1 | 1.E+06 | 1 |
599 | I | 3 | 2.E+06 | 2.E+06 | 1 | 2.E+06 | 1 | 1.E+06 | 1 |
600 | G | 3 | 4.E+03 | 5.E+03 | 1 | 4.E+03 | 1 | 3.E+03 | 1 |
601 | G | 3 | <300 | <300 | — | 3.E+02 | — | <300 | — |
602 | R | 3 | <300 | <300 | — | <300 | — | <300 | — |
603 | Y | 0 | <300 | <300 | — | <300 | — | <300 | — |
604 | V | 4 | 3.E+05 | >7E+5 | >2 | >7E+5 | >2 | >7E+5 | >2 |
605 | V | 4 | 5.E+05 | >7E+5 | >1 | >7E+5 | >1 | >7E+5 | >1 |
606 | A | 4 | <300 | <300 | — | 2.E+04 | >7 | 1.E+04 | >3 |
607 | G | 5 | 3.E+03 | 2.E+04 | 7 | 3.E+04 | 10 | 2.E+05 | 67 |
608 | K | 5 | <300 | 9.E+02 | >3 | 5.E+02 | >2 | 2.E+04 | >67 |
609 | M | 5 | <300 | <300 | — | <300 | — | 1.E+03 | >3 |
631 | A | 6 | 4.E+02 | 3.E+05 | 750 | 4.E+05 | 1,000 | 1.E+04 | 25 |
632 | L | 6 | <300 | <300 | — | 1.E+02 | — | 2.E+03 | >7 |
633 | T | 6 | <300 | <300 | — | <300 | — | <300 | — |
634 | E | 0 | 5.E+03 | 1.E+06 | 200 | >7E+5 | 140 | 4.E+05 | 80 |
635 | D | 7 | <300 | 3.E+02 | >1 | 3.E+04 | 100 | 3.E+02 | >1 |
636 | R | 7 | <300 | < 300 | — | 2.E+02 | — | <300 | — |
637 | L | 0 | 2.E+04 | >7E+5 | >35 | >7E+5 | >35 | 1.E+06 | 50 |
639 | R | 0 | 9.E+03 | >7E+5 | >78 | 1.E+06 | 111 | 7.E+04 | 8 |
640 | L | 0 | 1.E+05 | >7E+5 | >7 | >7E+5 | >7 | >7E+5 | 7 |
641 | E | 8 | >7E+5 | >7E+5 | — | >7E+5 | — | >7E+5 | — |
642 | E | 8 | 7.E+02 | 2.E+05 | 286 | 4.E+05 | 571 | 8.E+04 | 114 |
657 | V | 9 | >7E+5 | >7E+5 | — | >7E+5 | — | — | — |
658 | Y | 9 | >7E+5 | >7E+5 | — | >7E+5 | — | — | — |
659 | R | 9 | 1.3E+04 | >7E+5 | 54 | 2.4E+05 | 18 | — | — |
660 | R | 9 | 8.3E+02 | >7E+5 | 843 | 1.6E+04 | 19 | — | — |
661 | Y | 9 | 6.8E+02 | 1.1E+05 | 162 | 4.8E+02 | 1 | — | — |
662 | G | 9 | <300 | <300 | — | 5.1E+02 | >2 | — | — |
663 | E | 9 | <300 | <300 | — | <300 | — | — | — |
664 | E | 9 | <300 | <300 | — | <300 | — | — | — |
667 | N | 10 | 2.4E+05 | >7E+5 | >3 | — | — | >7E+5 | >3 |
668 | L | 10 | 2.8E+05 | >7E+5 | >3 | — | — | 7.E+05 | 3 |
669 | T | 10 | 4.9E+04 | 2.1E+05 | 4 | — | — | 7.4E+04 | 1 |
670 | R | 10 | 1.0E+03 | 1.4E+05 | 140 | — | — | 7.0E+04 | 70 |
672 | D | 10 | <300 | 2.3E+04 | >77 | — | — | 3.0E+04 | >100 |
673 | I | 10 | <300 | 6.4E+02 | >2 | — | — | 4.8E+02 | >2 |
674 | T | 10 | <300 | <300 | — | — | — | <300 | — |
675 | F | 10 | <300 | <300 | — | — | — | <300 | — |