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. 2015 Jul 22;112(31):9620–9625. doi: 10.1073/pnas.1512561112

Table S1.

H-D exchange protection factor (Pf) and Pf increase when bound to scFvs

Residue AA Switch NoAb Pf scFv4-20 Pf scFv4_20 ratio scFv4_16 Pf scFv4_16 ratio scFv3-1 Pf scFv3-1 ratio
585 I 0 >7E+5 >7E+5 >7E+5 >7E+5
586 A 0 >7E+5 >7E+5 >7E+5 >7E+5
587 N 1 >7E+5 >7E+5 >7E+5 >7E+5
588 H 1 2.E+04 3.E+05 15 2.E+05 10 8.E+04 4
589 R 1 2.E+04 1.E+05 5 5.E+04 3 4.E+05 20
591 L 1 3.E+03 8.E+04 27 2.E+04 7 9.E+04 30
592 F 1 <300 4.E+03 >13 4.E+03 >13 6.E+04 >200
593 T 2 >7E+5 >7E+5 >7E+5 >7E+5
594 H 2 5.E+05 >7E+5 >1 >7E+5 >1 >7E+5 >1
595 L 2 <300 1.E+03 >3 <300 1.E+03 >3
598 R 3 7.E+05 8.E+05 1 9.E+05 1 1.E+06 1
599 I 3 2.E+06 2.E+06 1 2.E+06 1 1.E+06 1
600 G 3 4.E+03 5.E+03 1 4.E+03 1 3.E+03 1
601 G 3 <300 <300 3.E+02 <300
602 R 3 <300 <300 <300 <300
603 Y 0 <300 <300 <300 <300
604 V 4 3.E+05 >7E+5 >2 >7E+5 >2 >7E+5 >2
605 V 4 5.E+05 >7E+5 >1 >7E+5 >1 >7E+5 >1
606 A 4 <300 <300 2.E+04 >7 1.E+04 >3
607 G 5 3.E+03 2.E+04 7 3.E+04 10 2.E+05 67
608 K 5 <300 9.E+02 >3 5.E+02 >2 2.E+04 >67
609 M 5 <300 <300 <300 1.E+03 >3
631 A 6 4.E+02 3.E+05 750 4.E+05 1,000 1.E+04 25
632 L 6 <300 <300 1.E+02 2.E+03 >7
633 T 6 <300 <300 <300 <300
634 E 0 5.E+03 1.E+06 200 >7E+5 140 4.E+05 80
635 D 7 <300 3.E+02 >1 3.E+04 100 3.E+02 >1
636 R 7 <300 < 300 2.E+02 <300
637 L 0 2.E+04 >7E+5 >35 >7E+5 >35 1.E+06 50
639 R 0 9.E+03 >7E+5 >78 1.E+06 111 7.E+04 8
640 L 0 1.E+05 >7E+5 >7 >7E+5 >7 >7E+5 7
641 E 8 >7E+5 >7E+5 >7E+5 >7E+5
642 E 8 7.E+02 2.E+05 286 4.E+05 571 8.E+04 114
657 V 9 >7E+5 >7E+5 >7E+5
658 Y 9 >7E+5 >7E+5 >7E+5
659 R 9 1.3E+04 >7E+5 54 2.4E+05 18
660 R 9 8.3E+02 >7E+5 843 1.6E+04 19
661 Y 9 6.8E+02 1.1E+05 162 4.8E+02 1
662 G 9 <300 <300 5.1E+02 >2
663 E 9 <300 <300 <300
664 E 9 <300 <300 <300
667 N 10 2.4E+05 >7E+5 >3 >7E+5 >3
668 L 10 2.8E+05 >7E+5 >3 7.E+05 3
669 T 10 4.9E+04 2.1E+05 4 7.4E+04 1
670 R 10 1.0E+03 1.4E+05 140 7.0E+04 70
672 D 10 <300 2.3E+04 >77 3.0E+04 >100
673 I 10 <300 6.4E+02 >2 4.8E+02 >2
674 T 10 <300 <300 <300
675 F 10 <300 <300 <300

Site resolution was obtained from the HDsite program (24). Protection factor (Pf) is HX slowing relative to calibrated reference rates (39). Numbers in boldface identify the values plotted in Fig. 3B.