Table S1.

Number of transfected cells in the experiment for silencing of a small subset of neurons

Freezing time, %	No. of mCherry+ cells	Total no. of cells
0.0	20	554
1.0	6	386
1.0	13	432
1.0	22	456
2.5	6	516
3.0	1	468
9.0	37	499
11.5	58	391
19.0	71	528
33.5	87	422
50.5	114	410
53.0	107	483

Cells were counted in a 500 μm × 500 μm area below the estimated position of the optical fiber tip from two 40-μm-thick slices per mouse.