Table S2.

Mutations detected in the CSF or tumor of each patient

Patient	Volume of CSF analyzed (mL)	DNA in CSF (ng)	Sequencing method	Mutation	Location	Gene	Transcript	Protein	cDNA	Mutant allele fraction in tumor (%)	Mutant allele fraction in CSF (%)
CGLI 02	4	245	WES	NM_144631.5 (ZNF513):c.413C > T	Exon	ZNF513	NM_144631.5	p.P138L	c.413C > T	49	Not detected
CGLI 03	7	314	WES	NM_181523.2 (PIK3R1):c.1690A > G	Exon	PIK3R1	NM_181523.2	p.N564D	c.1690A > G	39	0.1
CGLI 06	8	148	WES	NR_024604.1 (CLCA3P):c.446G > A	Exon	CLCA3P	NR_024604.1	p.R149Q	c.446G > A	36	0.2
CGLI 11	5	290	WES	NM_133450.3 (ANKS3):c.1738G > A	Exon	ANKS3	NM_133450.3	p.E580K	c.1738G > A	47	0.3
CGLI 12	5	71	WES	NM_003531.2 (HIST1H3C):c.83T > A	Exon	HIST1H3C	NM_003531.2	p.K28M	c.83T > A	44	0.2
CGLI 13	1.5	6	WES	NM_144965.1 (TTC16):c.1016T > A	Exon	TTC16	NM_144965.1	p.V339E	c.1016T > A	48	1.0
CGLI 14	7.5	187	Panel	NM_000546.5 (TP53):c.638G > A	Exon	TP53	NM_000546.5	p.R213Q	c.638G > A	59	19.6
CGLI 15	5	482	WES	NM_004990.3 (MARS):c.1487C > T	Exon	MARS	NM_004990.3	p.T496I	c.1487C > T	13	Not detected
CGLI 20	3	127	WES	NM_198229.2 (RGS12):c.2980C > T	Exon	RGS12	NM_198229.2	p.R994W	c.2980C > T	52	52.6
CGLI 22	5	8	WES	NM_001080522.2 (CC2D2A):c.1938G > A	Exon	CC2D2A	NM_001080522.2	p.W646X	c.1938G > A	54	Not detected
CGLI 25	10	253	WES	NM_001795.3 (CDH5):c.1211G > A	Exon	CDH5	NM_001795.3	p.S404N	c.1211G > A	50	0.3
CGLI 26	7	20	Panel	NM_005896.2 (IDH1):c.394C > T	Exon	IDH1	NM_005896.2	p.R132C	c.394C > T	43	0.3
CGLI 28	1	2,361	Panel	NM_005896.2 (IDH1):c.395G > A	Exon	IDH1	NM_005896.2	p.R132H	c.395G > A	33	33.0
CGLI 29	3.5	206	Panel	NM_000546.5 (TP53):c.742C > T	Exon	TP53	NM_000546.5	p.R248W	c.742C > T	69	0.2
CGLI 31	4	1,416	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	37	8.2
CGLI 35	2.5	432	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	46	1.4
CGLI 36	5.5	448	Panel	NM_000546.5 (TP53):c.742C > T	Exon	TP53	NM_000546.5	p.R248W	c.742C > T	65	14.3
CGLI 39	8.5	154	WES	NM_000268.3 (NF2):c.592C > T	Exon	NF2	NM_000268.3	p.R198X	c.592C > T	80	Not detected
CGLI 40	1.5	1,169	WES	NM_000388.3 (CASR):c.2549C > T	Exon	CASR	NM_000388.3	p.A850V	c.2549C > T	45	2.8
CGLI 41	4.25	87	Panel	NM_000546.5 (TP53):c.396G > C	Exon	TP53	NM_000546.5	p.K132N	c.396G > C	43	1.4
CGLI 42	0.75	20	WES	NM_001848.2 (COL6A1):c.1417G > A	Exon	COL6A1	NM_001848.2	p.G473R	c.1417G > A	39	0.5
CGLI 43	4.5	100	WES	NM_000268.3 (NF2):c.1009C > T	Exon	NF2	NM_000268.3	p.Q337*	c.1009C > T	61	Not detected
CGLI 44	1.5	64	WES	NM_001004439.1 (ITGA11):c.2140C > T	Exon	ITGA11	NM_001004439.1	p.R714*	c.2140C > T	16	Not detected
CGLI 47	6.8	29	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	44	22.1
CGLI 48	5	1,400	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	52	1.0
CGLI 50	5.6	820	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	47	18.8
CGLI 51	0.75	64	Panel	NM_198253.2 (TERT):c.1–124C > T	Promoter	TERT	NM_198253.2	NA	c.1–124C > T	10	5.9
CGLI 55	9	146	WES	NM_000314.4 (PTEN):c.388C > T	Exon	PTEN	NM_000314.4	p.R130*	c.388C > T	66	33.2
CGLI 56	6.5	1,155	WES	NM_014691.2 (AQR):c.1746delT	Exon	AQR	NM_014691.2	p.P582fs	c.1746delT	39	1.8
CGLI 58	9	90	WES	NM_052853.3 (ADCK2):c.1057T > A	Exon	ADCK2	NM_052853.3	p.F353I	c.1057T > A	23	Not detected
CGLI 60	8	1,335	WES	NM_004687.4 (MTMR4):c.2110C > G	Exon	MTMR4	NM_004687.4	p.L704V	c.2110C > G	40	18.7
CGLI 61	3	99	WES	NM_001122679.1 (TENM2):c.799G > A	Exon	TENM2	NM_001122679.1	p.A267T	c.799G > A	21	Not detected
CGLI 63	4	411	WES	NM_000264.3 (PTCH1):c.746+1C > A	Splice site	PTCH1	NM_000264.3	NA	c.746+1C > A	71	Not detected
CGLI 101	3.5	14	Panel	NM_000546.5 (TP53):c.731G > A	Exon	TP53	NM_000546.5	p.G244D	c.731G > A	36	0.9
CGLI 254	2.5	168	WES	NM_021140.2 (KDM6A):c.4153C > T	Exon	KDM6A	NM_021140.2	p.Q1385X	c.4153C > T	91	77.3

NA, not available; panel, directed sequencing of codons 130–139 of IDH1; codons 126–155, 144–178, and 250–262 of IDH2; all coding exons of TP53; and the TERT promoter (Materials and Methods).