Table 2.

Detection of CSF-tDNA using WES

Patient and sample type	Mutation	Genomic coordinate	Distinct coverage (SafeSeqS)	Mutant (%; SafeSeqS)	Distinct coverage (WES)	Mutant (%; WES)
CGLI 03
CSF	PIK3R1 p.N564D, c.A1690G	Chr5:67591097	57,921	0.1	76	0.0
Normal	PIK3R1 p.N564D, c.A1690G	Chr5:67591097	284	0.0	61	0.0
Primary tumor	PIK3R1 p.N564D, c.A1690G	Chr5:67591097	377	38.7	147	12.9
CGLI 29
CSF	TP53 p.R248W, c.C742T	Chr17:7577539	13,964	0.2	64	0.0
Normal	TP53 p.R248W, c.C742T	Chr17:7577539	NA	0.0	55	0.0
Primary tumor	TP53 p.R248W, c.C742T	Chr17:7577539	6,418	69.0	58	70.7
CGLI 36
CSF	TP53 p.R248W, c.C742T	Chr17:7577539	376,434	14.3	74	9.5
Normal	TP53 p.R248W, c.C742T	Chr17:7577539	57,818	0.0	75	0.0
Primary tumor	TP53 p.R248W, c.C742T	Chr17:7577539	44,981	65.0	25	72.0
CGLI 55
CSF	PTEN p.R130*, c.C388T	Chr10:89692904	251,609	33.2	63	42.9
Primary tumor	PTEN p.R130*, c.C388T	Chr10:89692904	91,515	65.9	56	66.1

Genomic coordinates refer to the human reference genome hg19 release (Genome Reference Consortium GRCh37, February of 2009). NA, not available.