Fig. S1.

MYD88 and TAK1 are essential for the EGF-induced activation of NFκB. (A, Upper) HME-BCL2 cells were infected with a vector encoding nontargeted shRNA (NTshRNA) or an shRNA against MYD88 and immunoblotted for MYD88 and β-actin expression. (Lower) The cells were serum starved for 24 h and then stimulated with 100 ng/mL EGF. Phosphorylated EGFR, IKK, IκB, ERK, and AKT were detected with phospho-specific antibodies. An anti-IκB antibody detected the degradation and resynthesis of this protein. (B, Upper) HME cells were infected with a vector encoding a scrambled shRNA (Con sh) or TAK1 shRNA. TAK1 was detected by the Western method. (Lower) EGF-starved cells were stimulated with EGF (100 ng/mL) for the indicated times or with IL-1β for 5 min.