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. 2015 Aug 12;10(8):e0133860. doi: 10.1371/journal.pone.0133860

Fig 1. Sea urchin regeneration assay.

Fig 1

A single strip of tube feet and adjacent spines from one ambulacral section were cut at the base (A, 1 day post amputation, dpa). Regrowth of spines was measured directly with calipers, and tube feet length was measured by image analysis of underwater photographs (B, 8 dpa), with the scale set from a ruler placed adjacent to cut section (visible on right, panel A). Panels C–E indicate regrowth in a control animal after 8, 15, and 22 dpa, respectively. Panels F–H indicate regrowth in an animal treated with 3 μg/g DAPT after 8, 15, and 22 dpa, respectively.