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Tube feet sampled 24 hours after final treatment with DAPT, 29 days post amputation. Gene expression (qRT-PCR) of selected Notch target genes (hey, gataC, hes, gcm), compared with the geometric mean of three most stable control genes (cyclophilin7, rpl8, profilin), data are geomeans ± s.e.m., n = 3 animals, *significant down-regulation (p < 0.05).
