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. 2015 Sep 1;405(1):82–95. doi: 10.1016/j.ydbio.2015.06.017

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 3 — E11.5 Chd7^fl/fl;Mesp1-Cre hearts show loss of the vestibular spine and misaligned endocardial cushions. (A) Transverse (TRANS) OPT sections through a Chd7^fl/fl heart showed formation of the primary atrial septum (green arrows) and the vestibular spine (red arrow). The endocardial superior and inferior AV cushions were seen in separate planes (yellow stars). In the Chd7^fl/fl;Mesp1-Cre heart (A′) the atrial septum formed as expected (green arrows), but the vestibular spine was completely absent (red star). The arrangement of the endocardial cushions was also grossly abnormal, with both seen adjacently in the same plane (yellow stars). (B) Digital re-slicing was used to produce coronal (COR) sections of the same hearts, which highlighted the abnormal positioning of the endocardial cushions following mesodermal Chd7 ablation (B′, yellow stars). (C) Morphology was also examined using H&E staining of coronal sections at E11.5, to better view the cushions of the OFT and AV canal. Despite their abnormal positioning within the AV canal, mesenchymal population of the cushions does not appear to be affected in mutants. Scale bars represent0.2 mm. oft indicates outflow tract; rv, right ventricle; lv, left ventricle; a, atrium; v, ventricle; avc, atrioventricular canal.