Table 3.
Genes identified through microarray analysis with differential expression in Chd7fl/fl;Mesp1-Cre hearts following multiple testing correction (log2 FC>0.5, adj.p.value <0.05).
| Ensembl ID | Symbol | Chrom | Description | log2 FC | p-Value | Adj.p.value |
|---|---|---|---|---|---|---|
| E11.5 | ||||||
| ENSMUSG00000041235 | Chd7 | 4 | Chromodomain helicase DNA binding protein 7 | −1.68 | 6.11E−09 | 0.000212 |
| ENSMUSG00000031558 | Slit2 | 5 | Slit homologue 2 (Drosophila) | −0.987 | 2.44E−08 | 0.000283 |
| ENSMUSG00000035551 | Igfbpl1 | 4 | Insulin-like growth factor binding protein-like | 1–1.30 | 1.08E−07 | 0.000939 |
| ENSMUSG00000019787 | Trdn | 10 | Triadin | −1.08 | 6.84E−07 | 0.00238 |
| ENSMUSG00000039579 | Grin3 a | 4 | Glutamate receptor ionotropic, NMDA3A | −0.972 | 6.21E−07 | 0.00238 |
| ENSMUSG00000028883 | Sema3a | 5 | Semaphorin 3A | −0.894 | 9.87E−07 | 0.00264 |
| ENSMUSG00000018893 | Mb | 15 | Myoglobin | −1.20 | 3.24E−06 | 0.00593 |
| ENSMUSG00000024868 | Dkk1 | 19 | Dickkopf homologue 1 (Xenopus laevis) | −1.23 | 1.13E−05 | 0.0127 |
| ENSMUSG00000004151 | Etv1 | 12 | ets Variant gene | 1–0.614 | 1.11E−05 | 0.0127 |
| ENSMUSG00000019906 | Lin7a | 10 | Lin-7 homologue A (C. elegans) | −1.57 | 1.80E−05 | 0.0179 |
| ENSMUSG00000044067 | Gpr22 | 12 | G protein-coupled receptor 22 | −0.839 | 2.11E−05 | 0.0188 |
| ENSMUSG00000052516 | Robo2 | 16 | Roundabout homologue 2 (Drosophila) | −0.907 | 2.38E−05 | 0.0201 |
| ENSMUSG00000074491 | Clec4g | 8 | C-type lectin domain family 4, member g | −0.986 | 2.52E−05 | 0.0203 |
| ENSMUSG00000069171 | Nr2f1 | 13 | Nuclear receptor subfamily 2, group F, member | 1–1.24 | 4.09E−05 | 0.0277 |
| ENSMUSG00000016494 | Cd34 | 1 | CD34 antigen | −0.647 | 4.14E−05 | 0.0277 |
| ENSMUSG00000073764 | Gm12888 | 4 | Predicted gene 12888 | −0.837 | 4.38E−05 | 0.0282 |
| ENSMUSG00000027463 | Slc52a3 | 2 | Solute carrier protein family 52, member | 3–1.37 | 4.54E−05 | 0.0287 |
| ENSMUSG00000007653 | Gabrb2 | 11 | Gamma-aminobutyric acid (GABA) A receptor, subunit beta | 2–0.821 | 5.02E−05 | 0.0292 |
| ENSMUSG00000020061 | Mybpc1 | 10 | Myosin binding protein C, slow-type | −1.25 | 5.32E−05 | 0.0303 |
| ENSMUSG00000025488 | Cox8b | 7 | Cytochrome c oxidase, subunit VIIIb | −1.17 | 6.16E−05 | 0.0323 |
| ENSMUSG00000020682 | Mmp28 | 11 | Matrix metallopeptidase 28 (epilysin) | −0.564 | 6.10E−05 | 0.0323 |
| ENSMUSG00000026678 | Rgs5 | 1 | Regulator of G-protein signalling | 5–2.04 | 6.76E−05 | 0.0340 |
| ENSMUSG00000049537 | Tecrl | 5 | Trans-2,3-enoyl-CoA reductase-like | −0.643 | 6.93E−05 | 0.0341 |
| ENSMUSG00000022519 | Srl | 16 | Sarcalumenin | −0.550 | 7.58E−05 | 0.0346 |
| ENSMUSG00000033737 | Fndc3c1 | X | Fibronectin type III domain containing 3C1 | −1.07 | 8.05E−05 | 0.0354 |
| ENSMUSG00000059742 | Kcnh7 | 2 | Potassium voltage-gated channel, subfamily H (eag-related), member 7 | −0.992 | 8.03E−05 | 0.0354 |
| ENSMUSG00000039057 | Myo16 | 8 | Myosin XVI | −0.717 | 0.000113 | 0.0446 |
| ENSMUSG00000055639 | Dach1 | 14 | Dachshund 1 (Drosophila) | −0.673 | 0.000117 | 0.0450 |
| ENSMUSG00000019851 | Perp | 10 | PERP, TP53 apoptosis effector | −0.650 | 0.000118 | 0.0450 |
| ENSMUSG00000061080 | Lsamp | 16 | Limbic system-associated membrane protein | −0.577 | 0.000116 | 0.0450 |
| ENSMUSG00000023328 | Ache | 5 | Acetylcholinesterase | −0.799 | 0.000137 | 0.0497 |
| ENSMUSG00000070867 | Trabd2b | 4 | Predicted gene 12824 | 0.860 | 2.19E−06 | 0.00475 |
| ENSMUSG00000021219 | Rgs6 | 12 | Regulator of G-protein signalling 6 | 0.895 | 4.07E−06 | 0.00615 |
| ENSMUSG00000029322 | Plac8 | 5 | Placenta-specific 8 | 1.82 | 5.56E−06 | 0.00773 |
| ENSMUSG00000025196 | Cpn1 | 19 | Carboxypeptidase N, polypeptide 1 | 1.47 | 6.39E−06 | 0.00854 |
| ENSMUSG00000022103 | Gfra2 | 14 | Glial cell line derived neurotrophic factor family receptor alpha 2 | 1.19 | 8.69E−06 | 0.0108 |
| ENSMUSG00000096555 | Olfr944 | 9 | Olfactory receptor 944 | 0.650 | 3.25E−05 | 0.0236 |
| ENSMUSG00000069583 | Krtap12-1 | 10 | Keratin associated protein 12-1 | 0.558 | 4.05E−05 | 0.0277 |
| ENSMUSG00000053519 | Kcnip1 | 11 | Kv channel-interacting protein 1 | 0.943 | 4.62E−05 | 0.0287 |
| ENSMUSG00000051251 | Nhlh1 | 1 | Nescient helix loop helix 1 | 0.676 | 5.73E−05 | 0.0321 |
| ENSMUSG00000095241 | Gm5478 | 15 | 0.732 | 5.85E−05 | 0.0322 | |
| ENSMUSG00000097451 | Rian | NA | NA | 0.746 | 8.35E−05 | 0.0363 |
| ENSMUSG00000027942 | 4933434E20Rik | 3 | RIKEN cDNA 4933434E20 gene | 1.73 | 9.34E−05 | 0.0396 |
| ENSMUSG00000031825 | Crispld2 | 8 | Cysteine-rich secretory protein LCCL domain containing 2 | 0.587 | 9.84E−05 | 0.0412 |
| ENSMUSG00000029309 | Sparcl1 | 5 | SPARC-like 1 | 0.723 | 0.000106 | 0.0433 |
| ENSMUSG00000070933 | Speer4d | 5 | Spermatogenesis associated glutamate (E)-rich protein 4d | 0.878 | 0.000110 | 0.0445 |
| ENSMUSG00000058952 | Cfi | 3 | Complement component factor i | 1.10 | 0.000113 | 0.0446 |
| ENSMUSG00000020427 | Igfbp3 | 11 | Insulin-like growth factor binding protein 3 | 0.854 | 0.000120 | 0.0453 |
| ENSMUSG00000040653 | Ppp1r14 c | 10 | Protein phosphatase 1, regulatory (inhibitor) subunit 14c | 0.586 | 0.000123 | 0.0461 |
| E13.5 | ||||||
| ENSMUSG00000041235 | Chd7 | 4 | Chromodomain helicase DNA binding protein 7 | −1.58 | 1.41E−08 | 0.000489 |
| ENSMUSG00000069171 | Nr2f1 | 13 | Nuclear receptor subfamily 2, group F, member | 1–1.43 | 8.43E−06 | 0.0366 |
| ENSMUSG00000023964 | Calcr | 6 | Calcitonin receptor | −1.39 | 1.04E−05 | 0.0403 |
| ENSMUSG00000073007 | Fam46d | X | Family with sequence similarity 46, member D | −1.11 | 1.24E−05 | 0.0425 |
| ENSMUSG00000033214 | Slitrk5 | 14 | SLIT and NTRK-like family, member | 5–0.670 | 1.35E−05 | 0.0425 |
| ENSMUSG00000028883 | Sema3 a | 5 | Semaphorin 3A | −0.704 | 1.63E−05 | 0.0437 |
| ENSMUSG00000025196 | Cpn1 | 19 | Carboxypeptidase N, polypeptide 1 | 1.93 | 2.32E−07 | 0.00404 |
| ENSMUSG00000055312 | 0610012H03Rik | 2 | RIKEN cDNA 0610012H03 gene | 0.911 | 3.92E−06 | 0.0273 |
| ENSMUSG00000069792 | Wfdc17 | 11 | Predicted gene 11428 | 1.61 | 7.76E−06 | 0.0366 |
Chrom. indicates chromosome. Fold changes are given on a binary logarithmic scale (log2 FC), P.values of significance were calculated using a Bayes moderated t test, whilst adj.p.values of significance were calculated using the Benjamini and Hochberg False Discovery Rate process. Numerical values are shown to 3 significant figures.