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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Glia. 2015 May 12;63(10):1825–1839. doi: 10.1002/glia.22847

Figure 3. Proliferation of OPCs is reduced by hyperoxia and improved by minocycline.

Figure 3

Immature oligodendroglia precursor cells undergo an intensive period of proliferation. To investigate this important step of oligodendroglia expansion we performed (A) immunohistochemistry of 10 μm cerebellar sections using antibodies against NG2 and the proliferation marker Ki67 to label proliferating OPCs at P7 immediately after 24h hyperoxia and after 2 and 4 days recovery in room air at P9 and at P11. (B) Numbers of NG2+Ki67+ OPCs in the cerebellar white matter were significantly reduced in rats after hyperoxia both at P7 and after recovery at P9 and P11. Administration of minocycline during hyperoxia abolished this reduction of OPC proliferation at all time points. (C) The total numbers of NG2+ cerebellar OPCs were not reduced after 24 h hyperoxia at P7. However, after recovery in room air at P9 and at P11, OPC numbers were significantly reduced in hyperoxia-experienced rats. Minocycline treatment during exposure to hyperoxia improved OPC numbers at both time points. Scale bar = 50μm. (**P<0.01 versus control, ##P<0.01 and #P<0.05, versus hyperoxia; ANOVA with consecutive t-test, n=4)