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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biotechnol Prog. 2015 Mar 1;31(4):990–996. doi: 10.1002/btpr.2062

Figure 4. Collagen matrix enhances the percentage of cells in G0/G1.

Figure 4

Cell cycle analysis was performed by flow cytometry using the hypotonic propidium iodide (PI) solution method. The cells were grown in three different conditions: 2D, 3D 0.15% collagen (low collagen), and 3D 0.30% collagen (high collagen). The results of 3 repeats (mean±SEM) are presented using a 2-tailed paired t test (*: 0.01<p<0.05, **: 0.001<p<0.01, n.s.: non-significant). Representative flow cytometry data showing distribution of relative DNA content of the cells are on the right.