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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Glia. 2015 Apr 27;63(10):1784–1796. doi: 10.1002/glia.22844

Figure 4. Immunofluorecence studies show lower GLT-1 and GLAST expression in neonatal cortex compared to hippocampus.

Figure 4

A. Confocal images of representative coronal sections stained for GLT-1 at 10x magnification. Scale bars are 500 μm. B. In GLT-1 stained sections, the fluorescence density from cortical ROIs normalized to the fluorescence density from CA1 stratum radiatum ROIs within each slice (P7, n = 3; P14, n = 4; P28, = 4). C. Examples from within ROIs in cortex and hippocampus at P28 showing the areas masked and excluded from the fluorescence density analysis of GLT-1 expression. Scale bars are 100 μm. D. Representative sections stained for GLAST. E. In GLAST stained sections, the fluorescence density from cortical ROIs normalized to the fluorescence density from CA1 stratum radiatum ROIs within each slice (P7, n = 4; P14, n = 4; P28, n = 3 cells). F. Examples from within regions of interest in cortex and hippocampus at P28 showing the areas masked and excluded from the fluorescence density analysis of GLAST expression. Scale bars are 100 μm. Error bars indicate SEM,*p<0.05, **p<0.01, ***p<0.001, one-sample t-test.