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. Author manuscript; available in PMC: 2016 Aug 12.
Published in final edited form as: Cell Rep. 2015 Feb 13;10(6):854–864. doi: 10.1016/j.celrep.2015.01.033

Figure 4. DDX28 Associates with the Large Mitoribosome Subunit.

Figure 4

(A–C) Sucrose gradient sedimentation analyses of DDX28 and mitoribosomal proteins from wild-type (WT) HEK293T mitochondrial extracts prepared in the presence of the indicated concentrations of (A) EDTA or MgCl2, (B) KCl, and (C) RNase A. The fractions were analyzed by immunoblotting using Abs against the indicated proteins.

(D) Immunoprecipitation of DDX28 from HEK293T mitochondrial extracts using an anti-DDX28 Image Ab (Sigma) as explained in the experimental procedures section.

(E) PCR analyses of reverse transcribed DDX28-co-immunoprecipitated RNA from UV-induced cross-linked mitochondria.

See also Fig S3.