Figure 5.

PRC2 Components and H3K27Me3 Are Enriched at Genes Downregulated in Dnmt3L^A/A SSCs

(A) Metagene plot of Suz12, Ezh2, and H3K27Me3 along the gene body (±4 kb of the transcription start site [TSS]) showing downregulated genes are more likely to be targets of the PRC2 complex. Plots show the average number of ChIP-seq sequence reads for Ezh2 and H3K27Me3 (input) in mouse ESCs at all genes (gray) and in genes upregulated (red) and downregulated (green) in DnmtL^A/A SSC. Sequence reads are plotted against the genomic distance from the gene TSS as reads per million total reads.

(B) Gene-body methylation plots of CG, CHG and CHH methylation at PcG target genes (blue line). Methylation plots at genes with low/weak expression in E16.5 PSG (red line) as well as all RefSeq genes (black line) are shown as controls. The data indicate a lack of specific enrichment of CG or non-CG methylation at PcG target genes in 1-dpp PSG.