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. 2015 Aug 12;10(8):e0135567. doi: 10.1371/journal.pone.0135567

Fig 1. Electrophoresis of GPCMV glycoprotein gene expression by RT-PCR assay.

Fig 1

Agarose gel analysis of RT-PCR products. Expression of viral glycoprotein genes GP73 (gN), GP74 (gO) and GP100 (gM) was investigated at various time points post infection. Input virus was wild type GPCMV (strain 22122) on GPL cells (moi = 1 pfu/cell). RT-PCR was performed as described in materials and methods. RT-PCR analysis of time point samples 0, 4, 8, 16, 24, 48 hr post infection indicated. Controls: C1, no template; C2, infected cell lysate no reverse transcriptase stage. GAPDH control RT-PCR for all time samples.