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. 2015 Aug 12;10(8):e0135567. doi: 10.1371/journal.pone.0135567

Fig 5. Transient expression constructs for GPCMV gH, gL, gO glycoproteins.

Fig 5

(i) Shows the structure of the C-terminal tagged ORFs and expression plasmids: gH (GP75) was tagged with GFP; gL (GP115) was tagged with mCherry; gO (GP74) both wild type and mutant ORFs were tagged with 3xFLAG tag. (ii) BLAST alignment of wild type and mutant gO ORFs. The 13 predicted N-glycosylation sites were knocked out in the mutant (gOdef) by substitution of an alanine (A) in place of threonine (T) to disrupt the N-glycosylation recognition sequence (N-X-T/S).