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. 2015 Aug 12;10(8):e0135567. doi: 10.1371/journal.pone.0135567

Fig 6. Transient expression of wild type or mutant gO in the presence or absence of GPCMV.

Fig 6

The cellular location and molecular weight of gO protein was investigated by transient expression studies. Panels E, J and O are western blots for wild type or mutant gO using anti-FLAG antibody. Other panels are immunofluorescene images of wild type and mutant gO protein cellular localization by transient plasmid expression in GPL cells (A-D); GPL cells the presence of tunicamycin (glycosylation inhibitor, 2.5 ug/ml) (F-I); GPL cells plus GPCMV (K-N). Matched paired panels for gO (FITC) or gO (FITC) and DAPI (merged): A and B; C and D; F and G; H and I; K and L; M and N. Western blots: for wild type or gO mutant (E); wild type or gO mutant in the presence of tunicamycin (J); wild type or gO mutant in the presence of GPCMV (O).