Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 12;10(8):e0135567. doi: 10.1371/journal.pone.0135567

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Coleman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 9 — All immunoprecipitations were performed with GFP-Trap (ChomoTek) as described in materials and methods. Proteins detected by specific antibodies to tags (GFP or FLAG). Transient expression assays were carried out in different plasmid combinations as indicated A-E. Samples shown for each combination are total cell lysate (Lys) and IP from total cell lysate. Control protein was GFP. gHGFP and gOdefFLAG IP in presence (A) or absence (B) of gLmCherry: A(i) gH western and A(ii) gO (def) western; B(i) gHwestern; (ii) gO (def) western. C. GFP and gO(def) FLAG control: C(i) GFPwestern; C(ii) gO(def) western. D. gHGFP and gO(wt) FLAG in the presence of gLmCherry and tunicamycin: D(i) gH western; D(ii) gOgO western. E. gHGFP and gO(wt) FLAG in presence of tunicamycin: E(i) gH western; E(ii) gOwestern.