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. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: Integr Biol (Camb). 2012 Dec;4(12):1498–1507. doi: 10.1039/c2ib20200j

Figure 7. Down-regulation of Runx2 inhibits the alkaline phosphatase activity in differentiating mouse mesenchymal progenitors cells (C2C12).

Figure 7

Transfection with Runx2 siRNA and BMP stimulation were as described in Figure 5. Cells were not transfected (A) and B), or transfected with 160 nM Runx2 siRNA sequence S-1 using C) Lipofectamine 2000 or D) DOTAP/siRNA/PPAA-g-1%Jeffamine and were induced to differentiate into osteblastic cell lineage with BMP-2 (B, C and D) after transfection. The histochemical analysis was performed on day 5 following treatemnt. E) Quantitative assay of alkaline phosphatase activity was performed using p-nitrophenyl phosphatase as a substrate. The asterisks represent statistical significant differences (p<0.05) in the alkaline phosphatase activity after transfection from that of untransfected control on days 3 and 4 post BMP-2 treatment respectively.