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. 2015 Aug 12;10(8):e0134907. doi: 10.1371/journal.pone.0134907

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — A. Schematic diagram for differentiating H1 hESCs into cortical neurons. B. Immunostaining [using antibodies reacting with stage-specific embryonic antigen-3 (SSEA3) and antigen-4 (SSEA-4)], nuclear staining [using 4',6-diamidino-2-phenylindole (DAPI) which is a fluorescent stain that binds strongly to A-T rich regions in DNA], and alkaline phosphatase (AP) staining (using a fluorescent substrate for AP for characterizing pluripotent stem cells) of H1 colonies before neuronal differentiation. C. Immunostaining of hESCs-derived neurons at days in dish (DIV) 32 using antibodies reacting with GABA and beta III tubulin, which are the biomarkers for GABA neurons.