Table 3. The expression levels of Bglap-rs1 and Chi3l4 genes by qPCR.
Sample | Average CT of target | Average CT of GAPDH | ΔCT a | ΔΔCT b | Relative to Mock |
---|---|---|---|---|---|
Bglap-rs1 | |||||
Mock | 22.81±0.09 | 18.80±0.03 | 4.00±0.09 | 0.00±0.09 | 1.00 |
5-FU administered | 22.95±0.02 | 18.26±0.04 | 4.68±0.05 | 0.67±0.05 | 0.62** |
Chi314 | |||||
Mock | 18.91±0.07 | 18.80±0.03 | 0.10±0.07 | 0.00±0.07 | 1.00 |
5-FU administered | 21.21±0.08 | 18.26±0.04 | 2.95±0.10 | 2.84±0.10 | 0.13** |
a The ΔCT value is determined by subtracting the average GAPDH CT value from the average target gene CT value. The standard deviation of the difference is calculated from the standard deviations of the target gene and GAPDH
b The calculation of ΔΔCT involves subtraction by the ΔCT calibrator value. This is a subtraction of an arbitrary constant, so the standard deviation of ΔΔCT is the same as the standard deviation of the ΔCT value
**p<0.01, compared with the control group (mock)