Skip to main content
. 2015 Aug 12;10(8):e0135102. doi: 10.1371/journal.pone.0135102

Table 3. The expression levels of Bglap-rs1 and Chi3l4 genes by qPCR.

Sample Average CT of target Average CT of GAPDH ΔCT a ΔΔCT b Relative to Mock
Bglap-rs1
  Mock 22.81±0.09 18.80±0.03 4.00±0.09 0.00±0.09 1.00
  5-FU administered 22.95±0.02 18.26±0.04 4.68±0.05 0.67±0.05 0.62**
Chi314
  Mock 18.91±0.07 18.80±0.03 0.10±0.07 0.00±0.07 1.00
  5-FU administered 21.21±0.08 18.26±0.04 2.95±0.10 2.84±0.10 0.13**

a The ΔCT value is determined by subtracting the average GAPDH CT value from the average target gene CT value. The standard deviation of the difference is calculated from the standard deviations of the target gene and GAPDH

b The calculation of ΔΔCT involves subtraction by the ΔCT calibrator value. This is a subtraction of an arbitrary constant, so the standard deviation of ΔΔCT is the same as the standard deviation of the ΔCT value

**p<0.01, compared with the control group (mock)