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. 2015 Apr 3;21(1):323–336. doi: 10.2119/molmed.2015.00018

Figure 2.

Figure 2

Identification of CHRFAM7A in THP1 cells. (A) 5′ RACE of human THP1 cells identified 11 of 14 CHRFAM7A transcripts sequenced as initiating at −446 bp from the CHRFAM7A open reading frame (A of the ATG = +1). (B) Translation of the DNA sequence established that the deduced CHRFAM7A protein has a unique 27 amino acid sequence that distinguishes CHRFAM7A from the amino terminus of CHRNA7. The CHRFAM7A exons and the amino acid sequence they encode are shown in blue and red. (C) The CHRNA7 gene encodes two protein variants that differ by the underlined sequence. (D) The common 386 amino acid sequence shared by both CHRFAM7A and CHRNA7 also shows the sequence encoded by the variant 2 of CHRFAM7A which is 100% identical between both genes but not detected in the RT-PCR experiments described here or observed after 5′RACE of THP1 cells.