Table 1.

Real-time PCR primer pairs. PCR primers used in the gene expression analysis

Gene name	Description	Primer sequence	Primer size (bp)
Refer-GAPDH	mGAPDH-F	5′-TGTGTCCGTCGTGGATCTGA-3′
	mGAPDH-R	5′-TTGCTGTTGAAGTCGCAGGAG-3′	150
CK	mck-F	5′-GAGATCTTCAAGAAGGCTGGTCA-3′
	mck-R	5′-GAGATGTCGAACACGGCG-3′	227
CRP	mcrp-F	5′-GCGGAAAAGTCTG-CACAAGG-3
	mcrp-R	5′-GGAGATAGCACAAAGTCCCACAT-3	153
ET-1	mET-1-F	5′-AGACCACAGACCAAGGGAACA-3′
	mET-1-R	5′-TCTGCTTGGCAGAAATTCCA-3′	392
ICAM-1	mICAM-1-F	5′-AGACACAAGCAAGAGAAGAAAAGG-3′
	mICAM-1-R	5′-TTGGGAACAAAGGTAGGAATGTAT-3′	425
MCP-1	mMCP-1-F	5′-GCTGACCCCAAGAAGGAATG-3′
	mMCP-1-R	5′-TTGAGGTGGTTGTGGAAAAGG-3′	184
NF-κB	mNF-κB-F	5′-GTGGAGGCATGTTCGGTAGTG-3′
	mN-κB-R	5′-TCTTGGCACAATCTTTAGGGC-3′	195
IκB	mIκB-F	5’-GGTGCAGGAGTGTTGGTGG-3′
	mIκB-R	5′-TGGCTGGTGTCTGGGGTAC-3′	173
IL-1β	m IL-1β-F	5′-GCTTCAGGCAGGCAGTATCA-3′
	mIL-1β-R	5′-TGCAGTTGTCTAATGGGAACG-3′	196
TNF-α	mTNF-α-F	5′-CCCTCCAGAAAAGACACCATG-3′
	mTNF-α-R	5′-CACCCCGAAGTTCAGTAGACAG-3′	183
TGF-β	mCcl21a-F	5′-CACGGTCCAACTCACAGGC-3′
	mCcl21a-R	5′-TTGAAGCAGGGCAAGGGT-3′	102
IL-4	mIL-4-F	5′-TGTAGGGCTTCCAAGGTGCT-3′
	mIL-4-R	5′-TGATGCTCTTTAGGCTTTCCAG-3′	199
IL-6	mIL-6-F	5′-GGGACTGATGCTGGTGACAAC-3′
	mIL-6-R	5′-CAACTCTTTTCTCATTTCCACGA-3′	163
STAT1	mSTAT1-F	5′-ACGCTGCCTATGATGTCTCG-3′
	mSTAT1-R	5′-ACGGGATCTTCTTGGAAGTTATC-3′	163
STAT3	mSTAT3-F	5′-TGACCAATAACCCCAAGAACG-3′
	mSTAT3-R	5′-TGACACCCTGAGTAGTTCACACC-3′	181
STAT6	mSTAT6-F	5′-AGCATCTTGCCGCACATCA-3′
	mSTAT6-R	5′-GGCAGGTGGCGGAACTCT-3′	128
GATA3	mGATA3-F	5′-CCACGGGAGCCAGGTATG-3′
	mGATA3-R	5′-CGGAGGGTAAACGGACAGAG-3′	169
GATA 4	mGata4-F	5′-CCTGGAAGACACCCCAATCT-3′
	mGata4-R	5′-GGTAGTGTCCCGTCCCATCT-3′	115
T-bet	mT-bet-F	5′-TGGACCCAACTGTCAACTGC-3′
	mT-bet-R	5′-CTCGGAACTCCGCTTCATAAC-3′	173