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. 2015 Jul 23;12(5):774–787. doi: 10.1016/j.celrep.2015.06.066

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

PCNA Accumulates on Chromatin in the Absence of the Replicative DNA Ligase Cdc9

(A) Outline of procedure for cell synchronization, induction of degradation of AID-tagged proteins, and sampling for chromatin fractionation.

(B) Flow cytometry analysis.

(C) In the absence of Elg1 (lane 4) or the DNA ligase Cdc9 (lane 6), PCNA accumulates on chromatin during DNA replication. Histone H3 is loading control. PCNA amounts on chromatin are indicated below, relative to no degron rad9Δ G1 sample (lane 1).

See also Figure S4.