Table 1.

Boltzmann parameters of activation relationships of wild-type hClC-1 channel and MC variants

[Cl−]i	Variants	N	V 0.5 (mV)	S (mV)	Min
134 mM	WT	11	−72.0 ± 3.3	47.2 ± 3.3	0.13 ± 0.02
	p.T82A	12	−74.2 ± 1.6	42.2 ± 1.5	0.08 ± 0.01
	p.R453W	13	−73.8 ± 1.7	34.1 ± 1.6	0.08 ± 0.01
	p.G190S	9	+116.2 ± 3.1	33.1 ± 2.1	0.11 ± 0.01
	p.G270V	13	+92.7 ± 5.9	52.6 ± 5.1	0.22 ± 0.01
	p.T82A + p.G190S	9	−22.1 ± 2.5	69.3 ± 2.9	0.06 ± 0.02
	p.R453W + p.G190S	6	+45.2 ± 1.9	56.4 ± 1.8	0.14 ± 0.01
4 mM	WT	14	−14.8 ± 2.4	43.5 ± 2.7	<0.01
	p.T82A	17	−4.1 ± 2.1	40.3 ± 2.3	<0.01
	p.R453W	12	−8.6 ± 3.0	56.3 ± 3.8	0.03 ± 0.01
	p.G270V	13	+84.4 ± 14.2	45.8 ± 8.3	0.07 ± 0.02

The averaged activation relationships obtained from n cells are fitted with a Boltzmann equation [P ₀(V) = Min + (1 − Min)/(1 + exp((V − V _0.5)/S)], where V _0.5 is the half-maximal activation potential, S is the slope factor, and Min is the minimal value of P ₀. The parameters are expressed as the calculated fit value ± the standard error of the fit