Figure 2.
DHX34 Interacts with NMD Effectors
(A) Cells were transiently transfected with FLAG-DHX34. Anti-FLAG IPs were performed in the presence or absence of RNase A. Inputs (1%) and anti-FLAG IPs (25%) were subjected to western analysis and probed for the indicated proteins.
(B) Immunoprecipitation of transiently transfected FLAG-DHX34 and FLAG-UPF1 from HEK293T cells in the absence or presence of RNase A. Inputs (0.5%) and anti-FLAG IPs (20%) were subjected to western analysis using the indicated antibodies.