Subject area |
Biology,Chemistry |
|
More specific subject area |
Microbial Proteomics, Bacterial physiology |
Type of data |
LC-ESI-MS/MS data |
How data was acquired |
Mass spectrometry |
Data format |
MS/MS data obtained from Orbitrap velos (Thermo scientific) was used for the identification of proteins. |
Experimental factors |
The protein of OMVs isolated form an Antarctic bacterium was identified using SEQUEST, MASCOT and PEAKS programs. The proteins commonly identified in all the three programs were accepted for further functional analysis. |
Experimental features |
In gel digested proteins with Trypsin were extracted with 5% TFA in 50% acetonitrile. Dried and desalted the peptides with Zip-tip C18 columns and loaded on Nano LC-ESI MS/MS.
|
Data source location |
CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India. |
Data accessibility |
Achieves of PRIDE, PXD 000221. The identified proteins were published in Journal of Proteome research [1]. |