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The potential target gene of mmu-miR-27a-5p, MCPIP1, is upregulated in macrophage stimulated with LPS. (a) LPS time-dependent mmu-miR-27a-5p expression. (b) Relative expression level of MCPIP1 mRNA under E. coli LPS stimulation for 6 h. (c) MCPIP1 protein expression under E. coli LPS stimulation. (d) Quantification of the western blots with BandScan 5.0 (n = 3).
