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. 2015 Aug 12;83(9):3693–3703. doi: 10.1128/IAI.00828-15

FIG 1.

FIG 1

Determination of the cell envelope localization of BB0744. (A) BB0744 is a soluble protein. B. burgdorferi 5A4 cells were partitioned into detergent (DET) and aqueous (AQ) phases with Triton X-114. Precipitated protein samples were resolved by SDS-PAGE and analyzed by Western blotting. OspA was included as a positive control for a membrane-localized protein (DET lane), and BB0796 was used as a positive control as an aqueous-phase protein (AQ lane). BB0744 was detected only in the aqueous phase. (B) BB0744 is not protease accessible in intact borrelial cells. B. burgdorferi 5A4 cells were suspended in PBS alone (Intact lanes) or PBS containing Triton X-100 (Triton X-100 lanes). Minus and plus signs signify the presence of added proteinase K (PK). The resulting B. burgdorferi samples were resolved by SDS-PAGE and analyzed by Western blotting. FlaB and OspC were used as controls for subsurface and surface-exposed proteins, respectively. No decrease in BB0744 was observed after proteinase K treatment. Lanes MW contained prestained protein molecular size markers (sizes are in kilodaltons).