FIG 2.

Surface exposure of BB0744. B. burgdorferi 5A4 cells were either washed or permeabilized by heat fixation prior to incubation with antibodies to the proteins listed on the right. Nonpermeabilized cells were then fixed to silylated slides with formaldehyde, and all cells were incubated with appropriate fluorescent secondary antibodies (anti-mouse Alexa 594 for mouse antibody to OspA, anti-chicken Alexa 647 for chicken antibody to FlaB, and anti-rat Alexa 488 for rat antibody to BB0744). FlaB served as a periplasmic protein control, and OspA was used as an outer surface protein control. BB0744 was labeled only in permeabilized cells. Scale bars, 10 μm.