FIG 5.
Loss of BB0744 affects the B. burgdorferi load in distal tissues. Quantitative PCR analysis of joint, lymph node, skin, and ear tissues was performed. Circles, ML23/pBBE22luc; triangles, SH100/pBBE22luc; squares, SH200/pBBE22luc. Copies of recA were quantified and represent the number of total B. burgdorferi genomes. To normalize the data to host tissue, β-actin copy numbers were also enumerated and data points are expressed as numbers of copies of B. burgdorferi genomes per 106 copies of β-actin. Bars indicate the average values of the samples tested. *, P < 0.05; **, P < 0.01; ***, P < 0.001.