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Overexpression of SdeA and SidJ. Saccharomyces strains were transformed with sdeA and sidJ expressed from a glucose-galactose-inducible expression system on pGML10. Transformed cells were plated on medium that repressed protein expression, i.e., SC −Leu with 2% glucose. Single colonies were then streaked onto repressing (glucose) and induction (galactose + raffinose) media. Plates were incubated at 30°C.
