TABLE 2.

Primers used in this study

Gene	Organism	Primer sequencea
narP	S. Typhimurium	5′-CCTGGAGCTCGTATTCGTCAATTACTG-3′
		5′-ACGGTCTAGAGTAGATTACGAATGTGC-3′
narL (upstream)	S. Typhimurium	5′-ATTTGTCGACCGCAGAAGATGTCACG-3′
		5′-CCACTCTAGACACCGTTGCTGGCTTCTCC-3′
narL (downstream)	S. Typhimurium	5′-CTGCTCTAGACGAAAGTACGGTCAAAGTG-3′
		5′-TACGTCGACTGGTCTTACGCAACAG-3′
napA (qPCR)b	S. Typhimurium	5′-TGAAAGAGAAAGGACCAGAAGCG-3′
		5′-TTGTTAGAGCGGAAACCAGCC-3′
narG (qPCR)	S. Typhimurium	5′-GAAGTGGAGTGGCGTGACAATG-3′
		5′-AGCGGATGAATAAACGGATGC-3′
16S rRNA	S. Typhimurium	5′-TGTTGTGGTTAATAACCGCA-3′
		5′-GACTACCAGGGTATCTAATCC-3′
Nos2	Mouse	5′-CCTCTTTCAGGTCACTTTGGTAGG-3′
		5′-TTGGGTCTTGTTCAGCCACGG-3′
Gapdh	Mouse	5′-TGTAGACCATGTAGTTGAGGTCA-3′
		5′-AGGTCGGTGTGAACGGATTTG-3′

^aUnderlined sequences indicate engineered restriction sites.

^bqPCR, quantitative PCR.