FIG 1.

Exacerbated mortality and bacterial dissemination in Hs2St-deficient mice. (A) Increased mortality of Hs2St-deficient mice after GBS challenge. Survival of mice was monitored for 8 days after intraperitoneal (i.p.) infection with 1.5 × 10⁸ CFU GBS. Data were pooled from two independent experiments (n = 19 for WT and n = 14 for Hs2St-deficient mice). Differences between groups were calculated by a log rank test. Mice were infected i.p. with 5 × 10⁷ CFU of GBS and then euthanized at 18 h postinfection for collection of organs for enumeration of bacterial CFU (B) and inflammatory cytokine ELISA (C). Data are medians with interquartile ranges from one of the two independent experiments. Each circle denotes one mouse (n = 6/group). Differences between groups were calculated by Mann-Whitney test (B and C).