FIG 3.

Effects of RevA deficiency on ankle thickness. Three- to 4-week-old female C3H/HeN mice were injected in the left footpad with 10³ (A) or 10⁴ (B) bacteria of strain B31-A3, the B31-A3ΔrevA1ΔrevA6 mutant, or the complemented strain. Ankle thickness was measured with calipers (triplicate measurements) at baseline and at 2 and 4 weeks postinfection. Two-way analysis of variance was used to calculate statistical differences between the groups injected with the parental strain or the B31-A3ΔrevA1ΔrevA6 mutant. For mice injected with 10³ bacteria (A), the difference was significant (P < 0.0001) at 4 weeks postinfection.