FIG 3.

K1-CPS and K2-CPS induce IL-1β and IL-18 secretion through NLRP3 inflammasome in macrophages. (A and B) Human THP-1 macrophages were incubated for 30 min with or without NLRP3 inhibitor glybenclamide (Glyb), for 5.5 h with or without K1-CPS or K2-CPS, and then for 30 min with or without ATP or nigericin. The levels of IL-1β (A) and IL-18 (B) in the culture medium were measured by ELISA and Western blotting, respectively. (C) sh-SC and sh-NLRP3 J774A.1 cells were incubated for 5.5 h with or without K1-CPS or K2-CPS and then for 30 min with or without ATP or nigericin. The levels of IL-1β in the culture medium were measured by ELISA. In panels A and C, the data are expressed as the means ± SD from three separate experiments. In panel B, the results are representative of three separate experiments. ***, P < 0.001.